Determining cell- and spatially-distinct skeletal muscle transcriptional aberrations in insulin resistance

Study Purpose: The purpose of this study is to look at gene expression changes to insulin stimulation in different cells and areas of skeletal muscle and how they are affected by obesity and insulin resistance in sedentary men and women between the ages of 30 and 65 years with; either a body mass index (BMI) between 18-27 kg/m2 or 30-40 kg/m2
Study Duration: 1-2 weeks
Study Commitment: 1 Screening visit
1 Outpatient visit
1 overnight inpatient visit
1 virtual follow-up
Age: 30-65 years old
Sex: Male & Female
Conditions/Eligibility: Sedentary men and women 30 – 65 years old, with BMI 18-27 kg/m2 or 30-40 kg/m2
Study Procedures: • Blood collections
• Urine Collections
• Pregnancy test (if applicable)
• Medical history and medication history
• AE assessment
• Anthropometrics (height, weight, BMI, waist, and hip measurements)
• Assessments of vitals (blood pressure, temperature, heart rate and respiratory rate)
• Electrocardiograms
• Oral glucose tolerance test
• Muscle blood flow
• DEXA scans
• Fitness assessments (VO2max exercise test)
• Physical Activity Monitoring
• Muscle tissue collections
• Glucose clamps
• Substrate use
Compensation: Up to $1000